Identifi cation of Metabolites in Malaria Infected Erythrocytes
نویسندگان
چکیده
As the premier vendor for metabolomics, Agilent Technologies has invested a great deal of effort to empower our customers with new metabolite identifi cation tools. This study demonstrates the use of Agilent’s new MS/MS spectral metabolomics library to elucidate the identifi cation of several amino acids that are important in malaria metabolism. Authors Theodore R. Sana, PhD Steven M. Fischer Cindy Lai Agilent Technologies, Inc. Santa Clara, CA, USA Dr. Sandra Chang Professor of Tropical Medicine John A. Burns School of Medicine, Honolulu, HI
منابع مشابه
A Q-TOF Generated, Metabolomics- Specifi c LC/MS/MS Library Facilitates Identifi cation of Metabolites in Malaria Infected Erythrocytes
As the premier vendor for metabolomics, Agilent Technologies has invested a great deal of effort to empower our customers with new metabolite identifi cation tools. This study demonstrates the use of Agilent’s new MS/ MS spectral metabolomics library to elucidate the identifi cation of several amino acids that are important in malaria metabolism. Authors Theodore R. Sana, PhD Steven M. Fischer ...
متن کاملAn LC/MS Metabolomics Discovery Workfl ow for Malaria-Infected Red Blood Cells Using Mass Profi ler Professional Software and LC-Triple Quadrupole MRM Confi rmation
The utility of a combined un-targeted discovery-based LC/MS and targeted confi rmation LC/MS/MS approach for mining metabolomics data was demonstrated using malaria-infected red blood cells as a model system. Several compounds, including arginine, citrulline, and ornithine, were identifi ed as metabolites whose biosynthesis was altered in erythrocytes infected by the malaria parasite. Authors T...
متن کاملExtraction of hydrophilic metabolites from Plasmodium falciparum-infected erythrocytes for metabolomic analysis.
Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics ...
متن کاملDrug Metabolite Purifi
Drug metabolism and pharmacokinetics (DMPK) studies require the identifi cation of drug metabolites usually by mass spectrometry (MS), and in later stages of drug development, confi rmation of metabolite structures by NMR. To achieve this, purifi cation of metabolites may be required. In this Application Note, purifi cation of bupropion drug metabolites was achieved using Agilent Automated Puri...
متن کاملPlasmodium falciparum-infected erythrocytes: effects of arylaminobenzoates
Following invasion of the human erythrocyte by the malaria parasite, Plasmodiumfalciparum, there appear in the parasitized cell new, high-capacity permeation pathways that transport a diverse range of low-molecular-mass solutes. In this study a series of 16 arylaminobenzoates, analogues of the Clchannel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), were tested for their effects on...
متن کامل